Ratiometric electrochemical detection of alkaline phosphatase.
نویسندگان
چکیده
A novel ferrocene-derived substrate for the ratiometric electrochemical detection of alkaline phosphatase (ALP) was designed and synthesised. It was demonstrated to be an excellent electrochemical substrate for the ALP-labelled enzyme-linked immunosorbent assay (ELISA).
منابع مشابه
A New Two-Photon Ratiometric Fluorescent Probe for Detecting Alkaline Phosphatase in Living Cells.
Alkaline phosphatase (ALP) is an important diagnostic indicator of many human diseases. To quantitatively track ALP in biosystems, herein, for the first time, we report an efficient two-photon ratiometric fluorescent probe, termed probe 1 and based on classic naphthalene derivatives with a donor-π-acceptor (D-π-A) structure and deprotection of the phosphoric acid moiety by ALP. The presence of ...
متن کاملScreen-printed microsystems for the ultrasensitive electrochemical detection of alkaline phosphatase.
Screen printing technique has been used to manufacture a microsystem where the graphite-based electrodes hold both a functional and an architectural task. The thick film manufacturing technique has proved valid to develop a very low volume (ca. 20 microL) device where different electrochemical operations can be very efficiently performed. Biomolecule immobilisation within the microsystem for bi...
متن کاملLsi-based Amperometric Chip Device with 400 Sensors for Detection of Alkaline Phosphatase and Respiration Activities of Embryonic Stem Cells
In this present study, we fabricated an LSI-based amperometric chip device with highly temporal and spacial resolution for electrochemical imaging, applied to detection of alkaline phosphatase (ALP) activity and respiration activity of embryonic stem (ES) cells.
متن کاملA novel aptasensor for thrombin detection based on alkaline phosphatase decorated ZnO/Pt nanoflowers as signal amplifiers.
To remedy the problems caused by the introduction of an additional electron mediator and realize signal amplification, a new strategy has been presented to construct an electrochemical aptasensor for thrombin detection based on the cascade electrocatalysis of alkaline phosphatase (ALP) and Pt nanoparticle (PtNP)-functionalized ZnO nanoflowers.
متن کاملDetection of an antibody to avian influenza virus by an electrochemical immunoassay (eELISA).
The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary ant...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 51 3 شماره
صفحات -
تاریخ انتشار 2015